Summary of Study ST001317
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000894. The data can be accessed directly via it's Project DOI: 10.21228/M84D6J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001317 |
| Study Title | Dynamics of Exposure, Phthalates, and Asthma in a Randomized Trial (DEPART) |
| Study Type | CHEAR Study |
| Study Summary | Project will investigate relationships between phthalate exposure, pediatric asthma health, and underlying biological pathways of toxicity among a rural, underserved Latino population located in Yakima Valley, WA. DEPART will benefit from the original study’s (HAPI’s) robust longitudinal repeat-measure design and community-engaged framework. DEPART will add new measurements including concentrations of urinary phthalate monoester metabolites and biomarkers of oxidative stress to better characterize exposure-response associations. This project’s primary goal is to deepen the understanding of pathophysiological phenomena underlying exposure-response relationships between phthalates and asthma health. Our specific aims are: (1) Characterize associations between urinary phthalate metabolite concentrations and short-term asthma morbidity, and (2) Determine individual relationships between urinary phthalate metabolite concentrations, short-term asthma morbidity, and biomarkers for oxidative stress to assess the potential for a mediating effect by oxidative stress. Covariates of interest will include atopic status, randomized intervention grouping, and the caregiver psychosocial stress assessment. |
| Institute | Icahn School of Medicine at Mount Sinai |
| Department | Department of Environmental Medicine and Public Health |
| Laboratory | Mount Sinai CHEAR Untargeted Laboratory Hub |
| Last Name | Walker |
| First Name | Douglas |
| Address | Atran Building RM AB3-39, 1428 Madison Ave |
| douglas.walker@mssm.edu | |
| Phone | 212-241-9891 |
| Submit Date | 2020-02-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002192 | AN002193 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um,100 Å) | Agilent Zorbax Eclipse Plus C18, RRHD (50 × 2.1 mm,1.8 um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Intensity | Peak Intensity |
Chromatography:
| Chromatography ID: | CH001607 |
| Chromatography Summary: | Sample extracts were analyzed using an ultra-high performance liquid chromatography (UHPLC) 1290 Infinity II system (including 0.3 µm inline filter, Agilent Technologies, Santa Clara, USA) with 1260 Infinity II isocratic pump (including 1:100 splitter) coupled to a 6550 iFunnel quadrupole-time time of flight (Q-TOF) mass spectrometer with a dual AJS electrospray ionization source (Agilent Technologies, Santa Clara, USA). Samples were maintained at 5C in the autosampler module. For polar metabolites separation, 2 uL of sample was injected onto a HILIC SeQuant® ZIC®-HILIC column (100 mm × 2.1 mm, 100 Å, 3.5 µm particle size, Merck, Darmstadt, Germany) with a guard fitting (14 mm × 1 mm, 5.0 µm particle size, Merck, Darmstadt, Germany) maintained at 25C. Separation occurred using Mobile phase A consisted of water with 0.1% formic acid and Mobile phase B consisted of Acetonitrile with 0.1% formic acid at a flow rate of 0.3 ml/min as described in Table 1. Data was acquired with a mass range of 40-1200 m/z. Solvent gradients were as follows: 95% solvent B, hold for 1.5 min; linear decrease to 40% solvent B at 12 minutes; hold for 2 min, linear decrease to 25% solvent B at 14.2 min, hold for 2.8 min; increase to 95% solvent B at 18 min, hold for 7 min. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um,100 Å) |
| Column Temperature: | 25C |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 25 min |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001608 |
| Chromatography Summary: | Sample extracts were analyzed using an ultra-high performance liquid chromatography (UHPLC) 1290 Infinity II system (including 0.3 µm inline filter, Agilent Technologies, Santa Clara, USA) with 1260 Infinity II isocratic pump (including 1:100 splitter) coupled to a 6550 iFunnel quadrupole-time time of flight (Q-TOF) mass spectrometer with a dual AJS electrospray ionization source (Agilent Technologies, Santa Clara, USA). Samples were maintained at 5C in the autosampler module. For nonpolar metabolites separation, 2 uL of sample sandwiched between 10 uL of water was injected onto a Zorbax Eclipse Plus C18, RRHD column (50 mm × 2.1 mm, 1.8 µm particle size, Agilent Technologies, Santa Clara, USA) coupled to a guard column (5 mm × 2 mm, 1.8 µm Agilent Technologies, Santa Clara, USA) maintained at 50C. Separation occurred using Mobile phase A consisted of water with 0.1% formic acid and Mobile phase B consisted of 2-propanol:ACN (90:10, v/v) with 0.1% formic acid at a flow rate of 0.4 ml/min as described in Table 2. Data was acquired with a mass range of 50-1200 m/z. Solvent gradients were as follows: 5% solvent B; linear increase to 98% solvent B at 13.5 min, hold for 1.5 min; decrease to 5% solvent B at 15.5 min, hold for 3.5 min. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent Zorbax Eclipse Plus C18, RRHD (50 × 2.1 mm,1.8 um) |
| Column Temperature: | 50C |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid |
| Analytical Time: | 19 min |
| Chromatography Type: | Reversed phase |
