Summary of Study ST002567
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001654. The data can be accessed directly via it's Project DOI: 10.21228/M8WX3D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002567 |
Study Title | Metabolomics of Human islets treated with DHT and high-glucose challenge |
Study Summary | Dihydrotestosterone (DHT) driven Androgen receptor activation in pancreatic beta-cells leads to the amplification of GLP-1R-mediated insulin exocytosis. Here we study the impact of DHT on the metabolic architecture of human pancreatic islets upon stimulation with DHT at high (16.7mM) and low (2.8mM) Glucose. |
Institute | Tulane University School of Medicine |
Last Name | Qadir |
First Name | Mirza Muhammad Fahd |
Address | SOM RM2301/Endocrinology |
mqadir@tulane.edu | |
Phone | 5049884646 |
Submit Date | 2023-04-17 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-19 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004228 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6530 qTOF |
Column | Agilent Zorbax Extend C18 (150 x 2.1mm, 1.8um) with matched guard column |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6530 QTOF |
Ion Mode | NEGATIVE |
Units | EicCoreArea/Peak Area |
Chromatography:
Chromatography ID: | CH003137 |
Methods Filename: | experiment_metadata |
Instrument Name: | Agilent 6530 qTOF |
Column Name: | Agilent Zorbax Extend C18 (150 x 2.1mm, 1.8um) with matched guard column |
Column Temperature: | 35°C |
Flow Gradient: | 0-2min hold 100/0/0 (%A/%B/%C); 2-12 min linear to 1/99/0, 12-20 min hold 1/99/0, 20-20.5 min linear to 5/0/95, 20.5-26.1 5/0/95, 26.1-26.3 min linear to 100/0/0, 26.3-33 min hold 100/0/0 |
Flow Rate: | 0-20.5 min hold 0.25mL/min, [18 min switch flow direction to backflush], 20.5-21.5 linear to 0.4mL/min, 21.5-22.3 linear to 0.8 mL/min, [26 min switch flow direction to forward] 26-26.1 linear to 0.6mL/min, 26.1-26.3 linear to 0.4 mL/min, 26.3-31.8 min hold 0.4mL/min, 31.8-32 min linear to 0.25mL/min |
Solvent A: | 97% water/3% methanol; 10 mM tributylamine; 5mM acetic acid |
Solvent B: | 100% methanol; 10 mM tributylamine; 5mM acetic acid |
Chromatography Type: | Reversed phase |