Summary of Study ST002567
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001654. The data can be accessed directly via it's Project DOI: 10.21228/M8WX3D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002567 |
| Study Title | Metabolomics of Human islets treated with DHT and high-glucose challenge |
| Study Summary | Dihydrotestosterone (DHT) driven Androgen receptor activation in pancreatic beta-cells leads to the amplification of GLP-1R-mediated insulin exocytosis. Here we study the impact of DHT on the metabolic architecture of human pancreatic islets upon stimulation with DHT at high (16.7mM) and low (2.8mM) Glucose. |
| Institute | Tulane University School of Medicine |
| Last Name | Qadir |
| First Name | Mirza Muhammad Fahd |
| Address | SOM RM2301/Endocrinology |
| mqadir@tulane.edu | |
| Phone | 5049884646 |
| Submit Date | 2023-04-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-19 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002674 |
| Sampleprep Summary: | Sample prep summary: Islet samples were extracted in 200μL of ice-cold 8:1:1 methanol:water:chloroform, with tissue disruption aided by immediate probe sonication for 20 seconds with a Branson 450 Sonifier set to output level 2, duty cycle 20%. The disrupted samples were allowed to incubate on ice for 10 minutes and were then centrifuged for 5 min at 14,000 xg to pellet cell debris. 180μL of supernatant were dried under a gentle stream of nitrogen gas at room temperature, and were then reconstituted in 45μL of 4:1 water:methanol and transferred to autosampler vials for analysis. Extraction method: Single-phase extraction using 8:1:1 methanol:water:chloroform Extract storage: -80°C until extraction; 4°C after reconstitution |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Single-phase extraction using 8:1:1 methanol:water:chloroform |
| Extract Storage: | -80℃ |
| Sample Resuspension: | -80°C until extraction; 4°C after reconstitution |
