Summary of Study ST002567
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001654. The data can be accessed directly via it's Project DOI: 10.21228/M8WX3D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002567 |
| Study Title | Metabolomics of Human islets treated with DHT and high-glucose challenge |
| Study Summary | Dihydrotestosterone (DHT) driven Androgen receptor activation in pancreatic beta-cells leads to the amplification of GLP-1R-mediated insulin exocytosis. Here we study the impact of DHT on the metabolic architecture of human pancreatic islets upon stimulation with DHT at high (16.7mM) and low (2.8mM) Glucose. |
| Institute | Tulane University School of Medicine |
| Last Name | Qadir |
| First Name | Mirza Muhammad Fahd |
| Address | SOM RM2301/Endocrinology |
| mqadir@tulane.edu | |
| Phone | 5049884646 |
| Submit Date | 2023-04-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004228 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6530 qTOF |
| Column | Agilent Zorbax Extend C18 (150 x 2.1mm, 1.8um) with matched guard column |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE |
| Units | EicCoreArea/Peak Area |
MS:
| MS ID: | MS003975 |
| Analysis ID: | AN004228 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS acquisition was performed in negative ion mode, scan rate 2 spectra/sec, mass range 50-1200 m/z. Source parameters were: drying gas temperature 250°C, drying gas flow rate 13 L/min, nebulizer pressure 35 psig, sheath gas temp 325°C and flow 12 l/min, capillary voltage 3500V, internal reference mass correction enabled. Data analysis was performed using Agilent Profinder 10.0 software in batch isotopologue extraction mode with automated natural isotope abundance correction enabled. Compound identification was performed by matching accurate mass and retention time to those of authentic standards analyzed using the same method. |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3500V |
| Dry Gas Flow: | 13L/min |
| Dry Gas Temp: | 250 C |
| Fragment Voltage: | 150V |
| Nebulizer: | 35 psig |
