Summary of Study ST002584

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001664. The data can be accessed directly via it's Project DOI: 10.21228/M8MH79 This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST002584
Study TitleMetabolomics analysis of ALDH1L1-expressing HuH7 cell lines.
Study SummaryOne-carbon metabolism is utilized for the de novo synthesis of purines. Given that tumor cells require large amounts of nucleotides, they are highly dependent on one-carbon metabolism. Among the one-carbon-metabolizing enzymes, the aldehyde dehydrogenase 1 family member L1 (ALDH1L1), which catalyzes 10-formyltetrahydrofolate to tetrahydrofolate, is considered a tumor suppressor gene, as its expression is reported to be attenuated or diminished in hepatocellular carcinoma. To clarify the effect of ALDH1L1 expression, we generated control and ALDH1L1-expressing HuH-7 (H7-1L1) cells, and measured the amounts of intracellular metabolites in both control and ALDH1L1-expressing by CE-TOFMS analysis.
Tohoku Medical and Pharmaceutical University
Last NameSasaki
First NameMasato
Address4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan
Submit Date2023-04-21
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-05-01
Release Version1
Masato Sasaki Masato Sasaki application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN004255 AN004256
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent CE Agilent CE
Column Agilent Fused silica capillary (80cm x 50um) Agilent Fused silica capillary (80cm x 50um)
MS instrument type Other Other
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Units Relative Area relative area


Chromatography ID:CH003161
Chromatography Summary:CE-TOFMS (ESI positive)
Instrument Name:Agilent CE
Column Name:Agilent Fused silica capillary (80cm x 50um)
Column Temperature:20
Flow Gradient:-
Flow Rate:+30 kV
Solvent A:1 M formic acid
Solvent B:-
Chromatography Type:CE
Chromatography ID:CH003162
Chromatography Summary:CE-TOFMS (ESI negative)
Instrument Name:Agilent CE
Column Name:Agilent Fused silica capillary (80cm x 50um)
Column Temperature:20
Flow Gradient:-
Flow Rate:−30 kV
Solvent A:50 mM ammonium acetate
Solvent B:-
Chromatography Type:CE