Summary of Study ST002584
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001664. The data can be accessed directly via it's Project DOI: 10.21228/M8MH79 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002584 |
| Study Title | Metabolomics analysis of ALDH1L1-expressing HuH7 cell lines. |
| Study Summary | One-carbon metabolism is utilized for the de novo synthesis of purines. Given that tumor cells require large amounts of nucleotides, they are highly dependent on one-carbon metabolism. Among the one-carbon-metabolizing enzymes, the aldehyde dehydrogenase 1 family member L1 (ALDH1L1), which catalyzes 10-formyltetrahydrofolate to tetrahydrofolate, is considered a tumor suppressor gene, as its expression is reported to be attenuated or diminished in hepatocellular carcinoma. To clarify the effect of ALDH1L1 expression, we generated control and ALDH1L1-expressing HuH-7 (H7-1L1) cells, and measured the amounts of intracellular metabolites in both control and ALDH1L1-expressing by CE-TOFMS analysis. |
| Institute | Tohoku Medical and Pharmaceutical University |
| Last Name | Sasaki |
| First Name | Masato |
| Address | 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan |
| msasaki@tohoku-mpu.ac.jp | |
| Phone | +81-22-727-0132 |
| Submit Date | 2023-04-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-01 |
| Release Version | 1 |
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Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Transgene |
|---|---|---|
| SA259165 | L1-1 | ALDH1L1 |
| SA259166 | L1-3 | ALDH1L1 |
| SA259167 | L1-2 | ALDH1L1 |
| SA259168 | C-3 | empty |
| SA259169 | C-1 | empty |
| SA259170 | C-2 | empty |
| Showing results 1 to 6 of 6 |
