Summary of Study ST002584
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001664. The data can be accessed directly via it's Project DOI: 10.21228/M8MH79 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002584 |
Study Title | Metabolomics analysis of ALDH1L1-expressing HuH7 cell lines. |
Study Summary | One-carbon metabolism is utilized for the de novo synthesis of purines. Given that tumor cells require large amounts of nucleotides, they are highly dependent on one-carbon metabolism. Among the one-carbon-metabolizing enzymes, the aldehyde dehydrogenase 1 family member L1 (ALDH1L1), which catalyzes 10-formyltetrahydrofolate to tetrahydrofolate, is considered a tumor suppressor gene, as its expression is reported to be attenuated or diminished in hepatocellular carcinoma. To clarify the effect of ALDH1L1 expression, we generated control and ALDH1L1-expressing HuH-7 (H7-1L1) cells, and measured the amounts of intracellular metabolites in both control and ALDH1L1-expressing by CE-TOFMS analysis. |
Institute | Tohoku Medical and Pharmaceutical University |
Last Name | Sasaki |
First Name | Masato |
Address | 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan |
msasaki@tohoku-mpu.ac.jp | |
Phone | +81-22-727-0132 |
Submit Date | 2023-04-21 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-05-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002692 |
Sampleprep Summary: | Cells were washed with 5%(w/w) mannitol solution, and then extracted with methanol. Extracts were filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome analysis. |