Summary of Study ST001775
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001129. The data can be accessed directly via it's Project DOI: 10.21228/M8RX1D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001775 |
| Study Title | Plasma metabolomics of diverse mouse strains infected with Plasmodium chabaudi |
| Study Summary | To uncover links between metabolism and disease severity in murine malaria, we performed plasma metabolomics via Metabolon on eight inbred, Plasmodium chabaudi-infected mouse strains with diverse disease phenotypes. We sacrificed and collected plasma from >=3 mice per strain per day of acute infection alongside uninfected control mice (approximately days 5-12 depending on mouse strain). We collected disease severity data, e.g. weight loss, liver enzymes, and anemia, concurrently. Together, these data enable 1) a picture of strain-specific and conserved metabolic responses during acute malaria, and 2) a comparison between metabolic responses and disease severity. |
| Institute | Stanford University |
| Department | Microbiology & Immunology |
| Laboratory | David Schneider |
| Last Name | Schneider |
| First Name | David |
| Address | 299 Campus Drive, Stanford, CA 94305 |
| dschneid@stanford.edu | |
| Phone | 650-724-8064 |
| Submit Date | 2021-04-11 |
| Num Groups | 8 mouse strains +/- Plasmodium infection |
| Total Subjects | 369 |
| Num Females | 369 |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-21 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001845 |
| Collection Summary: | See protocol document for full study details. Between 3 and 5 infected mice of each strain were euthanized each day from days 3-12 post-infection for cross-sectional analysis. Because the WSB/EiJ strain experiences delayed peak infection severity relative to the other mouse strains in this study, 3 or 4 WSB/EiJ mice were euthanized each day from days 3-17 post-infection for cross-sectional analysis. For each mouse strain, 2 uninfected control animals were euthanized at baseline and generally on odd-numbered days between days 3-12 or 3-17 for WSB/EiJ mice. Euthanasia was performed using carbon dioxide asphyxiation in accordance with Stanford University and APLAC guidelines for humane euthanasia. Following euthanasia, blood was collected via cardiac puncture using 25Gx5/8IN tuberculin syringes (Fisher Scientific 14-841-34). Syringes were primed by filling the syringe barrel with 0.5M EDTA, pH 8.0 anticoagulant and dispensing all but 50uL. Collected blood was stored on ice in 1.5mL Eppendorf tubes for 15-45 minutes before spinning at 1,000xg at 4 degrees C for 5 minutes in a tabletop centrifuge. Plasma was frozen at -80C immediately, and thawed/re-frozen once to aliquot for downstream cytokine, metabolite, and liver enzyme analyses. 100uL of plasma was shipped to Metabolon (https://www.metabolon.com/, Durham, NC, USA), which performed a combination of gas and liquid chromatography with mass spectrometry (GC/LC-MS). Compounds were identified by comparing sample peaks to an internal Metabolon library of known and unknown compounds. Raw peak values were obtained using area-the-curve. Additional data normalizations were performed by Metabolon to account for sample dilutions and day-to-day variation in instrument performance. |
| Collection Protocol Filename: | strainsmetabs_methods_for_mw.docx |
| Sample Type: | Blood (plasma) |
