Summary of Study ST001775
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001129. The data can be accessed directly via it's Project DOI: 10.21228/M8RX1D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001775 |
Study Title | Plasma metabolomics of diverse mouse strains infected with Plasmodium chabaudi |
Study Summary | To uncover links between metabolism and disease severity in murine malaria, we performed plasma metabolomics via Metabolon on eight inbred, Plasmodium chabaudi-infected mouse strains with diverse disease phenotypes. We sacrificed and collected plasma from >=3 mice per strain per day of acute infection alongside uninfected control mice (approximately days 5-12 depending on mouse strain). We collected disease severity data, e.g. weight loss, liver enzymes, and anemia, concurrently. Together, these data enable 1) a picture of strain-specific and conserved metabolic responses during acute malaria, and 2) a comparison between metabolic responses and disease severity. |
Institute | Stanford University |
Department | Microbiology & Immunology |
Laboratory | David Schneider |
Last Name | Schneider |
First Name | David |
Address | 299 Campus Drive, Stanford, CA 94305 |
dschneid@stanford.edu | |
Phone | 650-724-8064 |
Submit Date | 2021-04-11 |
Num Groups | 8 mouse strains +/- Plasmodium infection |
Total Subjects | 369 |
Num Females | 369 |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-21 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN002882 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | ion counts |
MS:
MS ID: | MS002675 |
Analysis ID: | AN002882 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Proprietary analytical software for integration and peak picking. Units are scaled imputed ion counts, derived from raw ion counts and corrected by Metabolon to account for day-to-day variation in instrument performance. Directly from Metabolon: "Values for each sample are normalized by volume and dilution effect. ; Each biochemical in OrigScale is then rescaled to set the median equal to 1. ; Lastly, missing values are imputed with the minimum." |
Ion Mode: | POSITIVE |