Summary of Study ST000093

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000085. The data can be accessed directly via it's Project DOI: 10.21228/M8JS3X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000093
Study TitleMetabolomics Analysis of Frontal Fibrosing Alopecia
Study TypeUnaffected and Affected patient scalp biopsies
Study SummaryScarring alopecia consists of a collection of disorders characterized by destruction of hair follicles, replacement with fibrous scar tissue, and irreversible hair loss. Alopecia affects men and women worldwide and can be a significant source of psychological stress and depression for affected individuals. The purpose of this study was to explore metabolic profiles in scalp tissue samples from normal control subjects (n=6) and in matched samples obtained from affected (n=12) and unaffected (n=12) areas of the scalp in patients with lymphocytic Frontal Fibrosing Alopecia (FFA). Frontal fibrosing alopecia results from destruction of hair follicles by an inflammatory lymphocytic infiltrate that is localized around the upper portion of the hair follicle.
Institute
Case Western Reserve University
DepartmentDermatology
LaboratoryKarnik Lab
Last NameKarnik
First NamePratima
Emailpsk11@case.edu
Phone216-368-0209
Submit Date2014-07-24
Num Groups3 groups-Paired unaffected and affected (n=12),Normals(n=6)
Total SubjectsPatients (N=12), Normals (N=6)
Study CommentsAffected scalp biopsies were obtained from frontal scalp, Unaffected from occipital scalp. Normal scalp biopsies were obtained from the occipital scalp.
Raw Data AvailableNo
Analysis Type DetailGC-MS/LC-MS
Release Date2014-07-26
Release Version1
Pratima Karnik Pratima Karnik
https://dx.doi.org/10.21228/M8JS3X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000147 AN000148 AN000149
Analysis type MS MS MS
Chromatography type GC GC GC
Chromatography system
Column GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles
MS Type EI ESI ESI
MS instrument type Single quadrupole Single quadrupole Single quadrupole
MS instrument name Thermo Trace DSQ Thermo LTQ Thermo LTQ
Ion Mode POSITIVE POSITIVE NEGATIVE
Units Unspecified Unspecified Unspecified

MS:

MS ID:MS000123
Analysis ID:AN000147
Instrument Name:Thermo Trace DSQ
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:For GC/MS analysis, samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization.
Ion Mode:POSITIVE
Capillary Temperature:60°C to 340°C
  
MS ID:MS000124
Analysis ID:AN000148
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer.
Ion Mode:POSITIVE
Capillary Temperature:60°C to 340°C
  
MS ID:MS000125
Analysis ID:AN000149
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer.
Ion Mode:NEGATIVE
Capillary Temperature:60°C to 340°C
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