Summary of Study ST000093

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000085. The data can be accessed directly via it's Project DOI: 10.21228/M8JS3X This work is supported by NIH grant, U2C- DK119886.


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Study IDST000093
Study TitleMetabolomics Analysis of Frontal Fibrosing Alopecia
Study TypeUnaffected and Affected patient scalp biopsies
Study SummaryScarring alopecia consists of a collection of disorders characterized by destruction of hair follicles, replacement with fibrous scar tissue, and irreversible hair loss. Alopecia affects men and women worldwide and can be a significant source of psychological stress and depression for affected individuals. The purpose of this study was to explore metabolic profiles in scalp tissue samples from normal control subjects (n=6) and in matched samples obtained from affected (n=12) and unaffected (n=12) areas of the scalp in patients with lymphocytic Frontal Fibrosing Alopecia (FFA). Frontal fibrosing alopecia results from destruction of hair follicles by an inflammatory lymphocytic infiltrate that is localized around the upper portion of the hair follicle.
Case Western Reserve University
LaboratoryKarnik Lab
Last NameKarnik
First NamePratima
Submit Date2014-07-24
Num Groups3 groups-Paired unaffected and affected (n=12),Normals(n=6)
Total SubjectsPatients (N=12), Normals (N=6)
Study CommentsAffected scalp biopsies were obtained from frontal scalp, Unaffected from occipital scalp. Normal scalp biopsies were obtained from the occipital scalp.
Raw Data AvailableNo
Analysis Type DetailGC-MS/LC-MS
Release Date2014-07-26
Release Version1
Pratima Karnik Pratima Karnik application/zip

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Sample Preparation:

Sampleprep ID:SP000108
Sampleprep Summary:Metabolon’s standard solvent extraction method. The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS.
Sampleprep Protocol Filename:Metabolon_Methods_CASE-03-11VW.docx
Sample Derivatization:50?L for GC/MS analysis using equal parts bistrimethyl-silyl-trifluoroacetamide and solvent mixture