Summary of Study ST000890
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000619. The data can be accessed directly via it's Project DOI: 10.21228/M8NT1F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000890 |
| Study Title | Identification of RXR Ligands |
| Study Type | Idenfication of Ligands by HPLC-MS |
| Study Summary | Free fatty acids in mouse plasma were identified and quantified by LC-MS. Through differential feeding and PHZ (phnylhydrazine) dosing, coupled with mass spectrometry, we identified the long chain fatty acid C24:5 as a natural RXRA ligand, which was dynamically increased in concentration in response to hematopoietic stress. Collectively, these data demonstrate that natural RXRA ligands are present and are dynamically regulated in vivo in mouse hematopoietic cells. |
| Institute | Washington University in St. Louis |
| Department | Diabetic Cardiovascular Disease Center, School of Medicine |
| Laboratory | Metabolomics Core |
| Last Name | Fujiwara |
| First Name | Hideji |
| Address | 660 South Euclid Ave, St. Louis MO 63110 |
| hfujiwar@wustl.edu | |
| Phone | 314-747-0494 |
| Submit Date | 2017-09-22 |
| Study Comments | Units of measurement:peak area ratio: analyte peak area/peak area of internal standard |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000619 |
| Project DOI: | doi: 10.21228/M8NT1F |
| Project Title: | Endogenous Retinoid X Receptor Ligands in Mouse Hemotopoietic Cells |
| Project Type: | MS Identification and Quantification of Ligands |
| Project Summary: | The retinoid X receptor α (RXRA) has been implicated in diverse hematological processes. However, it is unknown whether natural ligands of RXRA are present or regulated in hematopoietic cells. We quantified lipids in the serum of mice treated with a vitamin A deficient diet, a fatty acid deficient diet, and following phenylhydrazine treatment. In parallel, these serum samples were applied to RXRA reporter cells to identify conditions which contained increased concentrations of natural RXRA ligands. Mass spectrometry quantification of serum lipids was correlated with data from the RXRA reporter cells to identify serum lipids that increased in conditions associated with augmented RXRA ligand concentrations in the RXRA reporter assay. |
| Institute: | Washington University in St. Louis |
| Department: | Diabetic Cardiovascular Disease Center |
| Laboratory: | Metabolomics Core |
| Last Name: | Fujiwara |
| First Name: | Hideji |
| Address: | 660 South Euclide Avenue, St. Lois, MO 63110 |
| Email: | hfujiwar@wustl.edu |
| Phone: | 314-747-0494 |
| Funding Source: | NIH R01 HL128447, NIH P30 DK020579 |
| Publications: | Science Signaling 2018, being accepted for publication |
| Contributors: | Haixia Niu, Hideji Fujiwara, Orsola di Martino, Gayla Hadwiger, Thomas E. Frederick, María P. Menéndez-Gutiérrez, Mercedes Ricote, Gregory R. Bowman, John S. Welch |
