Summary of Study ST002153

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001366. The data can be accessed directly via it's Project DOI: 10.21228/M85115 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST002153
Study TitleData from plasma metabolome analysis of APP-KI and Wild type mice treated with B. breve MCC1274
Study SummaryRecently, we showed that administration of B. breve MCC1274 reduced amyloid-beta production, inhibited microglial activation, and suppressed inflammatory responses in the brains of APP knock-in (AppNL-G-F) mice. To elucidate the mechanism of action of this probiotic strain in an Alzheimer's disease-like model, we orally fed 3-month-old mice with B. breve MCC1274 or saline for 4 months and comprehensively investigated metabolites in plasma using CE-FTMS and LC-TOFMS.
Morinaga milk industry CO., LTD.
DepartmentNext generation science institute
LaboratoryFrontier research section
Last NameOhno
First NameKazuya
Address1-83 5-Chome Higashihara, Zama-city, Kanagawa-Pref. Japan
Submit Date2022-04-18
Analysis Type DetailLC-MS
Release Date2022-10-03
Release Version1
Kazuya Ohno Kazuya Ohno application/zip

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP002245
Sampleprep Summary:For CE-FTMS, 40 µL of plasma was added to 160 µL of methanol containing internal standards at 0°C, 120 µL of Milli-Q water was added and mixed thoroughly, and 240 µL of this mixture was added to a Millipore 5 kDa cutoff filter (ULTRAFREE MC PLHCC, HMT) at 9100 × g for 120 min at 4°C to remove macromolecules. The filtrate was evaporated to dryness under vacuum, dissolved in 40 µL Milli-Q water, and donated to HMT's ω Scan package using capillary electrophoresis Fourier transform mass spectrometry (CE-FTMS).For LC-TOFMS, 80 µL of plasma was added to 240 µL of 1% formic acid/acetonitrile containing internal standards at 0ºC. The mixture was centrifuged at 2,300 x g, 4ºC for 5 min, then centrifuged at 2,300 x g, 4ºC for 5 min, followed by centrifugation of a hybrid SPE phospholipid cartridge (30 mg/mL of hybrid SPE-phospholipid, 30 mg/mL of (SUPELCO) and filtered to remove phospholipids. The filtrate was then evaporated to dryness under nitrogen, dissolved in 80 μL of 50% isopropanol (v/v), and metabolomic analysis was performed using liquid chromatography time-of-flight mass spectrometry (LC-TOFMS).