Summary of study ST001413

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000969. The data can be accessed directly via it's Project DOI: 10.21228/M8FD5Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001413
Study TitleMetabolomic and Transcriptomic Signatures of Prenatal Excessive Methionine in Mice
Study TypeMS Analysis
Study SummaryMicronutrients are key regulators of prenatal one-carbon (C1) metabolism. We show here that prenatal excessive methionine (MET), a principal micronutrient and methyl-donor, produces in early life stages significant changes in the components of brain C1 pathways and other metabolic pathways including glutamate transmission, mitochondrial function, and lipid metabolism.
Institute
University of California, Irvine
DepartmentDepartment of Pharmaceutical Sciences and Department of Computer Science
LaboratoryAmal Alachkar Group. Pierre Baldi Group
Last NameChen
First NameSiwei
Address66304 Verano Place Rd. South
Emailsiweic@uci.edu
Phone2066585923
Submit Date2020-06-24
Num Groups4
Total Subjects28
Num Males28
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2020-07-24
Release Version1
Siwei Chen Siwei Chen
https://dx.doi.org/10.21228/M8FD5Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000969
Project DOI:doi: 10.21228/M8FD5Z
Project Title:Metabolic and Transcriptomic Signatures of Prenatal Excessive Methionine in Mice
Project Type:MS Analysis
Project Summary:Micronutrients are key regulators of prenatal one-carbon (C1) metabolism. In this study, we show here that prenatal excessive methionine (MET), a principal micronutrient and methyl-donor, produces in early life stages significant changes in the components of brain C1 pathways and other metabolic pathways including glutamate transmission, mitochondrial function, and lipid metabolism.
Institute:University of California, Irvine
Department:Department of Pharmaceutical Sciences, School of Medicine; Department of Computer Science, School of Information and Computer Sciences
Laboratory:Amal Alachkar Group; Pierre Baldi Group
Last Name:Chen
First Name:Siwei
Address:66304 Verano Place Rd. South
Email:siweic@uci.edu
Phone:2066585923

Subject:

Subject ID:SU001487
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:newborn mice of 24hr old or adult mice of 13 week old
Gender:Male
Animal Animal Supplier:Charles River Laboratories (Wilmington, MA)

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Condition Age
SA115960Adult2Control 13 weeks
SA115961Adult1Control 13 weeks
SA115962Adult6Control 13 weeks
SA115963Adult5Control 13 weeks
SA115964Adult3Control 13 weeks
SA115965Adult4Control 13 weeks
SA115966Newborn4Control 24 hour
SA115967Newborn5Control 24 hour
SA115968Newborn2Control 24 hour
SA115969Newborn8Control 24 hour
SA115970Newborn7Control 24 hour
SA115971Newborn6Control 24 hour
SA115972Newborn3Control 24 hour
SA115973Newborn1Control 24 hour
SA115974Adult7Methionine 13 weeks
SA115975Adult8Methionine 13 weeks
SA115976Adult9Methionine 13 weeks
SA115977Adult12Methionine 13 weeks
SA115978Adult11Methionine 13 weeks
SA115979Adult10Methionine 13 weeks
SA115980Newborn15Methionine 24 hour
SA115981Newborn16Methionine 24 hour
SA115982Newborn14Methionine 24 hour
SA115983Newborn10Methionine 24 hour
SA115984Newborn9Methionine 24 hour
SA115985Newborn11Methionine 24 hour
SA115986Newborn12Methionine 24 hour
SA115987Newborn13Methionine 24 hour
Showing results 1 to 28 of 28

Collection:

Collection ID:CO001482
Collection Summary:Metabolic profiling on whole homogenate brain tissues collected from 24 hours or 13 weeks’ old mice was carried out at the West Coast metabolics center (University of California Davis).
Sample Type:Brain
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001502
Treatment Summary:Swiss Webster mice, 8-9 weeks’ age, were obtained from Charles River Laboratories (Wilmington, MA). One male and two female mice were group-housed and maintained on a 12- hours light/dark cycle (light on at 7:00 am) with food and water available ad libitum. Pregnant mice were individually housed from the thirteen day of pregnancy (gestational day 13) until delivery. Pregnant mice that mated with the same male mouse were subcutaneously administered with L-Methionine (MET) at a dose of 750 mg/kg (Sigma-Aldrich) or saline (SAL) twice a day for seven to eight consecutive days from gestational day 14 until delivery.

Sample Preparation:

Sampleprep ID:SP001495
Sampleprep Summary:Metabolic profiling on whole homogenate brain tissues collected from 24 hours or 13 weeks’ old mice was carried out at the West Coast metabolics center (University of California Davis).
Processing Storage Conditions:-80℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN002363
Analysis type MS
Chromatography type HILIC
Chromatography system SCIEX 6600TTOF
Column Thermo Accucore HILIC (100 x 2.1mm, 2.6um)
MS Type APCI
MS instrument type Triple TOF
MS instrument name ABI Sciex 6600 TripleTOF
Ion Mode POSITIVE
Units m/z

Chromatography:

Chromatography ID:CH001733
Instrument Name:SCIEX 6600TTOF
Column Name:Thermo Accucore HILIC (100 x 2.1mm, 2.6um)
Chromatography Type:HILIC

MS:

MS ID:MS002205
Analysis ID:AN002363
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:APCI
MS Comments:None
Ion Mode:POSITIVE
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