Summary of Study ST002567

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001654. The data can be accessed directly via it's Project DOI: 10.21228/M8WX3D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002567
Study TitleMetabolomics of Human islets treated with DHT and high-glucose challenge
Study SummaryDihydrotestosterone (DHT) driven Androgen receptor activation in pancreatic beta-cells leads to the amplification of GLP-1R-mediated insulin exocytosis. Here we study the impact of DHT on the metabolic architecture of human pancreatic islets upon stimulation with DHT at high (16.7mM) and low (2.8mM) Glucose.
Institute
Tulane University School of Medicine
Last NameQadir
First NameMirza Muhammad Fahd
AddressSOM RM2301/Endocrinology
Emailmqadir@tulane.edu
Phone5049884646
Submit Date2023-04-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-04-19
Release Version1
Mirza Muhammad Fahd Qadir Mirza Muhammad Fahd Qadir
https://dx.doi.org/10.21228/M8WX3D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001654
Project DOI:doi: 10.21228/M8WX3D
Project Title:Metabolomics of Human islets treated with DHT and high-glucose challenge
Project Summary:Dihydrotestosterone (DHT) driven Androgen receptor activation in pancreatic beta-cells leads to the amplification of GLP-1R-mediated insulin exocytosis. Here we study the impact of DHT on the metabolic architecture of human pancreatic islets upon stimulation with DHT at high (16.7mM) and low (2.8mM) Glucose.
Institute:Tulane University
Last Name:Qadir
First Name:Mirza Muhammad Fahd
Address:SOM RM2301/Endocrinology, 333 S, Liberty, New Orleans, LA, 70112, USA
Email:mqadir@tulane.edu
Phone:5049884646

Subject:

Subject ID:SU002668
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA2580232021-10-01_010_D1_E116.7G_DHT_30min
SA2580242021-11-29_051_D1_E316.7G_DHT_30min
SA2580252021-10-01_014_D2_E116.7G_DHT_30min
SA2580262021-10-01_034_D3_E116.7G_DHT_30min
SA2580272021-11-29_010_D2_E216.7G_DHT_30min
SA2580282021-11-29_006_D1_E216.7G_DHT_30min
SA2580292021-11-29_049_D2_E316.7G_DHT_30min
SA2580302021-11-29_017_D3_E216.7G_DHT_30min
SA2580312021-11-29_032_D3_E316.7G_DHT_30min
SA2580322021-11-29_005_H2_E216.7G_DHT_60min
SA2580332021-10-01_012_H3_E116.7G_DHT_60min
SA2580342021-11-29_043_H1_E316.7G_DHT_60min
SA2580352021-10-01_019_H2_E116.7G_DHT_60min
SA2580362021-11-29_018_H3_E216.7G_DHT_60min
SA2580372021-10-01_026_H1_E116.7G_DHT_60min
SA2580382021-11-29_031_H2_E316.7G_DHT_60min
SA2580392021-11-29_024_H1_E216.7G_DHT_60min
SA2580402021-11-29_035_H3_E316.7G_DHT_60min
SA2580412021-11-29_044_B2_E316.7G_EtoH_30min
SA2580422021-11-29_038_B1_E316.7G_EtoH_30min
SA2580432021-11-29_012_B1_E216.7G_EtoH_30min
SA2580442021-11-29_019_B2_E216.7G_EtoH_30min
SA2580452021-11-29_053_B3_E316.7G_EtoH_30min
SA2580462021-10-01_025_B1_E116.7G_EtoH_30min
SA2580472021-10-01_016_B3_E116.7G_EtoH_30min
SA2580482021-10-01_031_B2_E116.7G_EtoH_30min
SA2580492021-11-29_008_B3_E216.7G_EtoH_30min
SA2580502021-11-29_034_F2_E316.7G_EtoH_60min
SA2580512021-11-29_011_F1_E216.7G_EtoH_60min
SA2580522021-11-29_036_F3_E316.7G_EtoH_60min
SA2580532021-11-29_033_F1_E316.7G_EtoH_60min
SA2580542021-10-01_022_F1_E116.7G_EtoH_60min
SA2580552021-10-01_013_F2_E116.7G_EtoH_60min
SA2580562021-11-29_022_F2_E216.7G_EtoH_60min
SA2580572021-10-01_035_F3_E116.7G_EtoH_60min
SA2580582021-11-29_025_F3_E216.7G_EtoH_60min
SA2580592021-11-29_047_C2_E32.8G_DHT_30min
SA2580602021-11-29_054_C3_E32.8G_DHT_30min
SA2580612021-11-29_046_C1_E32.8G_DHT_30min
SA2580622021-11-29_026_C2_E22.8G_DHT_30min
SA2580632021-10-01_023_C3_E12.8G_DHT_30min
SA2580642021-10-01_032_C1_E12.8G_DHT_30min
SA2580652021-10-01_021_C2_E12.8G_DHT_30min
SA2580662021-11-29_027_C3_E22.8G_DHT_30min
SA2580672021-11-29_021_C1_E22.8G_DHT_30min
SA2580682021-11-29_037_G3_E32.8G_DHT_60min
SA2580692021-11-29_007_G1_E22.8G_DHT_60min
SA2580702021-10-01_011_G3_E12.8G_DHT_60min
SA2580712021-11-29_015_G2_E22.8G_DHT_60min
SA2580722021-11-29_040_G2_E32.8G_DHT_60min
SA2580732021-10-01_029_G2_E12.8G_DHT_60min
SA2580742021-11-29_004_G3_E22.8G_DHT_60min
SA2580752021-11-29_039_G1_E32.8G_DHT_60min
SA2580762021-10-01_015_G1_E12.8G_DHT_60min
SA2580772021-10-01_027_A3_E12.8G_EtoH_30min
SA2580782021-10-01_033_A2_E12.8G_EtoH_30min
SA2580792021-11-29_013_A2_E22.8G_EtoH_30min
SA2580802021-11-29_050_A1_E32.8G_EtoH_30min
SA2580812021-10-01_028_A1_E12.8G_EtoH_30min
SA2580822021-11-29_041_A2_E32.8G_EtoH_30min
SA2580832021-11-29_030_A3_E32.8G_EtoH_30min
SA2580842021-11-29_023_A3_E22.8G_EtoH_30min
SA2580852021-11-29_014_A1_E22.8G_EtoH_30min
SA2580862021-11-29_009_E1_E22.8G_EtoH_60min
SA2580872021-10-01_020_E3_E12.8G_EtoH_60min
SA2580882021-10-01_024_E2_E12.8G_EtoH_60min
SA2580892021-10-01_017_E1_E12.8G_EtoH_60min
SA2580902021-11-29_028_E2_E22.8G_EtoH_60min
SA2580912021-11-29_020_E3_E22.8G_EtoH_60min
SA2580922021-11-29_048_E2_E32.8G_EtoH_60min
SA2580932021-11-29_052_E1_E32.8G_EtoH_60min
SA2580942021-11-29_045_E3_E32.8G_EtoH_60min
Showing results 1 to 72 of 72

Collection:

Collection ID:CO002661
Collection Summary:Following the incubation period, tubes were briefly centrifuged at low speed and media was withdrawn. 150mM ammonium acetate in water were added to rinse residual media (150mM ammonium acetate is an osmolarity-matching, mass spectrometry compatible rinse buffer with near-neutral pH). Tubes were centrifuged, supernatant was withdrawn, and they were frozen by immersion in liquid nitrogen and stored at -80C prior to extraction.
Sample Type:Pancreas
Volumeoramount Collected:500-1000uL
Storage Conditions:-80℃
Storage Vials:1ml Eppendorf Tubes
Collection Tube Temp:-40C
Additives:Ammonium acetate 150mM

Treatment:

Treatment ID:TR002680
Treatment Summary:Human islets were treated overnight with 10nM DHT or EtOH in a humidified incubator containing 4% CO2 at 37°C and then batched in groups of 100 islets in microcentrifuge tubes and treated for either 30 or 60 minutes with media containing either 2.8mM or 16.7 mM 13C6 glucose; the same DHT or EtOH concentrations were maintained.
Treatment Compound:DHT
Treatment Dose:10nM
Treatment Dosevolume:1ul per 1ml media
Treatment Doseduration:24 hours prior to experiment
Treatment Vehicle:Ethanol

Sample Preparation:

Sampleprep ID:SP002674
Sampleprep Summary:Sample prep summary: Islet samples were extracted in 200μL of ice-cold 8:1:1 methanol:water:chloroform, with tissue disruption aided by immediate probe sonication for 20 seconds with a Branson 450 Sonifier set to output level 2, duty cycle 20%. The disrupted samples were allowed to incubate on ice for 10 minutes and were then centrifuged for 5 min at 14,000 xg to pellet cell debris. 180μL of supernatant were dried under a gentle stream of nitrogen gas at room temperature, and were then reconstituted in 45μL of 4:1 water:methanol and transferred to autosampler vials for analysis. Extraction method: Single-phase extraction using 8:1:1 methanol:water:chloroform Extract storage: -80°C until extraction; 4°C after reconstitution
Processing Storage Conditions:-80℃
Extraction Method:Single-phase extraction using 8:1:1 methanol:water:chloroform
Extract Storage:-80℃
Sample Resuspension:-80°C until extraction; 4°C after reconstitution

Combined analysis:

Analysis ID AN004228
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 6530 qTOF
Column Agilent Zorbax Extend C18 (150 x 2.1mm, 1.8um) with matched guard column
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode NEGATIVE
Units EicCoreArea/Peak Area

Chromatography:

Chromatography ID:CH003137
Methods Filename:experiment_metadata
Instrument Name:Agilent 6530 qTOF
Column Name:Agilent Zorbax Extend C18 (150 x 2.1mm, 1.8um) with matched guard column
Column Temperature:35°C
Flow Gradient:0-2min hold 100/0/0 (%A/%B/%C); 2-12 min linear to 1/99/0, 12-20 min hold 1/99/0, 20-20.5 min linear to 5/0/95, 20.5-26.1 5/0/95, 26.1-26.3 min linear to 100/0/0, 26.3-33 min hold 100/0/0
Flow Rate:0-20.5 min hold 0.25mL/min, [18 min switch flow direction to backflush], 20.5-21.5 linear to 0.4mL/min, 21.5-22.3 linear to 0.8 mL/min, [26 min switch flow direction to forward] 26-26.1 linear to 0.6mL/min, 26.1-26.3 linear to 0.4 mL/min, 26.3-31.8 min hold 0.4mL/min, 31.8-32 min linear to 0.25mL/min
Solvent A:97% water/3% methanol; 10 mM tributylamine; 5mM acetic acid
Solvent B:100% methanol; 10 mM tributylamine; 5mM acetic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003975
Analysis ID:AN004228
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS acquisition was performed in negative ion mode, scan rate 2 spectra/sec, mass range 50-1200 m/z. Source parameters were: drying gas temperature 250°C, drying gas flow rate 13 L/min, nebulizer pressure 35 psig, sheath gas temp 325°C and flow 12 l/min, capillary voltage 3500V, internal reference mass correction enabled. Data analysis was performed using Agilent Profinder 10.0 software in batch isotopologue extraction mode with automated natural isotope abundance correction enabled. Compound identification was performed by matching accurate mass and retention time to those of authentic standards analyzed using the same method.
Ion Mode:NEGATIVE
Capillary Voltage:3500V
Dry Gas Flow:13L/min
Dry Gas Temp:250 C
Fragment Voltage:150V
Nebulizer:35 psig
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