Summary of Study ST002153

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001366. The data can be accessed directly via it's Project DOI: 10.21228/M85115 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST002153
Study Title	Data from plasma metabolome analysis of APP-KI and Wild type mice treated with B. breve MCC1274
Study Summary	Recently, we showed that administration of B. breve MCC1274 reduced amyloid-beta production, inhibited microglial activation, and suppressed inflammatory responses in the brains of APP knock-in (AppNL-G-F) mice. To elucidate the mechanism of action of this probiotic strain in an Alzheimer's disease-like model, we orally fed 3-month-old mice with B. breve MCC1274 or saline for 4 months and comprehensively investigated metabolites in plasma using CE-FTMS and LC-TOFMS.
Institute	Morinaga milk industry CO., LTD.
Department	Next generation science institute
Laboratory	Frontier research section
Last Name	Ohno
First Name	Kazuya
Address	1-83 5-Chome Higashihara, Zama-city, Kanagawa-Pref. Japan
Email	kazuya-oono443@morinagamilk.co.jp
Phone	81462523067
Submit Date	2022-04-18
Analysis Type Detail	LC-MS
Release Date	2022-10-03
Release Version	1

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Project:

Project ID:	PR001366
Project DOI:	doi: 10.21228/M85115
Project Title:	Improvement of cognitive function by B. breve MCC1274 and elucidation of its mechanism of action
Project Summary:	Although many studies have suggested that probiotics could modulate various brain functions via the gut-brain axis, little is known about the mechanism underlying this process. We previously reported the beneficial effects of a probiotic strain, Bifidobacterium breve MCC1274, on cognitive function in preclinical and clinical studies. Recently, we showed that administration of this strain reduced amyloid-beta production, inhibited microglial activation, and suppressed inflammatory responses in the brains of APP knock-in (AppNL-G-F) mice. To elucidate the mechanism of action of this probiotic strain in an Alzheimer's disease-like model, we orally fed 3-month-old mice with B. breve MCC1274 or saline for 4 months and comprehensively investigated metabolites in plasma using CE-FTMS and LC-TOFMS.
Institute:	Morinaga milk industry CO., LTD.
Department:	Next generation science institute
Laboratory:	Frontier research section
Last Name:	Ohno
First Name:	Kazuya
Address:	1-83 5-Chome Higashihara, Zama-city, Kanagawa-Pref. Japan
Email:	kazuya-oono443@morinagamilk.co.jp
Phone:	81462523067

Subject:

Subject ID:	SU002239
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	AppNL-G-F or wild type mice
Age Or Age Range:	7 months old
Gender:	Male and female
Animal Animal Supplier:	RIKEN Center for Brain Science
Animal Light Cycle:	12 hours light and dark cycle
Species Group:	Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Model	Intervention
SA206357	AT-1	Alzheimer’s disease model	B. breve MCC1274
SA206358	AT-2	Alzheimer’s disease model	B. breve MCC1274
SA206359	AT-3	Alzheimer’s disease model	B. breve MCC1274
SA206360	AT-4	Alzheimer’s disease model	B. breve MCC1274
SA206361	AC-3	Alzheimer’s disease model	Saline
SA206362	AC-4	Alzheimer’s disease model	Saline
SA206363	AC-2	Alzheimer’s disease model	Saline
SA206364	AC-1	Alzheimer’s disease model	Saline
SA206365	WT-1	Wild type	B. breve MCC1274
SA206366	WT-3	Wild type	B. breve MCC1274
SA206367	WT-2	Wild type	B. breve MCC1274
SA206368	WT-4	Wild type	B. breve MCC1274
SA206369	WC-2	Wild type	Saline
SA206370	WC-3	Wild type	Saline
SA206371	WC-4	Wild type	Saline
SA206372	WC-1	Wild type	Saline

Showing results 1 to 16 of 16

Showing results 1 to 16 of 16

Collection:

Collection ID:	CO002232
Collection Summary:	Plasma samples were obtained from AD-like model and wild-type mice orally administered B. breve MCC1274 or saline for 4 months.
Sample Type:	Blood (plasma)
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR002251
Treatment Summary:	Three-month-old AppNL-G-F and wild type mice were randomly assigned to a vehicle group and a probiotic group. The vehicle group received saline, and the probiotic group received B. breve MCC1274 (1 × 10＾9 cfu/200 μl saline/mouse) orally 5 times a week for 4 months.
Treatment Compound:	Bifidobacterium breve MCC1274
Treatment Route:	oral administration
Treatment Dosevolume:	1 × 10＾9 cfu/200 μl saline
Treatment Vehicle:	saline

Sample Preparation:

Sampleprep ID:	SP002245
Sampleprep Summary:	For CE-FTMS, 40 µL of plasma was added to 160 µL of methanol containing internal standards at 0°C, 120 µL of Milli-Q water was added and mixed thoroughly, and 240 µL of this mixture was added to a Millipore 5 kDa cutoff filter (ULTRAFREE MC PLHCC, HMT) at 9100 × g for 120 min at 4°C to remove macromolecules. The filtrate was evaporated to dryness under vacuum, dissolved in 40 µL Milli-Q water, and donated to HMT's ω Scan package using capillary electrophoresis Fourier transform mass spectrometry (CE-FTMS).For LC-TOFMS, 80 µL of plasma was added to 240 µL of 1% formic acid/acetonitrile containing internal standards at 0ºC. The mixture was centrifuged at 2,300 x g, 4ºC for 5 min, then centrifuged at 2,300 x g, 4ºC for 5 min, followed by centrifugation of a hybrid SPE phospholipid cartridge (30 mg/mL of hybrid SPE-phospholipid, 30 mg/mL of (SUPELCO) and filtered to remove phospholipids. The filtrate was then evaporated to dryness under nitrogen, dissolved in 80 μL of 50% isopropanol (v/v), and metabolomic analysis was performed using liquid chromatography time-of-flight mass spectrometry (LC-TOFMS).

Sampleprep ID:

SP002245

Sampleprep Summary:

For CE-FTMS, 40 µL of plasma was added to 160 µL of methanol containing internal standards at 0°C, 120 µL of Milli-Q water was added and mixed thoroughly, and 240 µL of this mixture was added to a Millipore 5 kDa cutoff filter (ULTRAFREE MC PLHCC, HMT) at 9100 × g for 120 min at 4°C to remove macromolecules. The filtrate was evaporated to dryness under vacuum, dissolved in 40 µL Milli-Q water, and donated to HMT's ω Scan package using capillary electrophoresis Fourier transform mass spectrometry (CE-FTMS).For LC-TOFMS, 80 µL of plasma was added to 240 µL of 1% formic acid/acetonitrile containing internal standards at 0ºC. The mixture was centrifuged at 2,300 x g, 4ºC for 5 min, then centrifuged at 2,300 x g, 4ºC for 5 min, followed by centrifugation of a hybrid SPE phospholipid cartridge (30 mg/mL of hybrid SPE-phospholipid, 30 mg/mL of (SUPELCO) and filtered to remove phospholipids. The filtrate was then evaporated to dryness under nitrogen, dissolved in 80 μL of 50% isopropanol (v/v), and metabolomic analysis was performed using liquid chromatography time-of-flight mass spectrometry (LC-TOFMS).

Combined analysis:

Analysis ID	AN003526	AN003527
Chromatography ID	CH002604	CH002605
MS ID	MS003284	MS003285
Analysis type	MS	MS
Chromatography type	Normal phase	Reversed phase
Chromatography system	Agilent 1260	Agilent 1200
Column	fused silica capillary (50 μm i.d. × 80 cm total length; Polymicro Technologies, Inc., Phoenix, AZ)	ODS
MS Type	ESI	ESI
MS instrument type	Orbitrap	TOF
MS instrument name	Thermo Q Exactive Plus Orbitrap	Agilent 6210 TOF
Ion Mode	UNSPECIFIED	UNSPECIFIED
Units	peak area	peak area

Chromatography:

Chromatography ID:	CH002604
Instrument Name:	Agilent 1260
Column Name:	fused silica capillary (50 μm i.d. × 80 cm total length; Polymicro Technologies, Inc., Phoenix, AZ)
Internal Standard:	H3304-1002, Human Metabolome Technologies, Inc. (HMT), Tsuruoka, Yamagata, Japan
Solvent A:	commercial electrophoresis buffer (H3301-1001; I3302-1023 for cation; anion analyses, respectively, HMT)
Chromatography Type:	Normal phase

Chromatography ID:	CH002605
Instrument Name:	Agilent 1200
Column Name:	ODS
Internal Standard:	H3304-1002, Human Metabolome Technologies, Inc. (HMT), Tsuruoka, Yamagata, Japan
Chromatography Type:	Reversed phase

MS:

MS ID:	MS003284
Analysis ID:	AN003526
Instrument Name:	Thermo Q Exactive Plus Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	The spectrometer was scanned from m/z 60 to 900 in positive mode, and from m/z 70 to 1,050 in negative mode
Ion Mode:	UNSPECIFIED

MS ID:	MS003285
Analysis ID:	AN003527
Instrument Name:	Agilent 6210 TOF
Instrument Type:	TOF
MS Type:	ESI
MS Comments:	The spectrometer was scanned from m/z 50 to 1,000 and peaks were extracted using MasterHands, automatic integration software (Keio University, Tsuruoka, Yamagata, Japan)
Ion Mode:	UNSPECIFIED